科研成果

Genetic characteristics and linkage of virulence genes of the Puccinia striiformis f. sp. tritici TSA-6 isolate to Yr5 host resistance

作者:  来源:  发布日期:2022-07-25  浏览次数:

论文信息:Genetic characteristics and linkage of virulence genes of the  Puccinia striiformis f. sp.  tritici TSA-6 isolate to  Yr5 host resistance. Gensheng Zhang, Wei Liu, Lin Wang, Meng Ju, Xiaxia Tian, Zhimin Du, Zhensheng Kang*, and Jie Zhao*. Plant Disease. 2022. Doi: https://doi.org/10.1094/PDIS-07-22-1637-RE

JCR分区Q1,中科院大类二区,TOP期刊,IF: 4.614

论文摘要: To understand inheritance of the TSA-6  Puccinia striiformis f. sp.  tritici  isolate, virulent to  Yr5 , that was recently detected in China, we analyzed avirulence and virulence of 120 selfed progeny lines from Berberis shensiana. The results showed that the TSA-6 isolate is virulent against the  Yr5  resistance gene, and overall progeny lines were categorized into 73 virulence phenotypes (VPs), and of these, 72 VPs differed from the isolate TSA-6, and only 1 VP including 3 progeny was identical to the parental isolate. The analyses indicated that the TSA-6 isolate is homozygous for avirulence at  Yr10 ,  Yr15  and  Yr26  resistance loci, and virulence at  YrA  resistance locus. The TSA-6 isolate is heterozygous for avirulence at the  Yr2 ,  Yr3 ,  Yr5 ,  Yr7  and Yr8 resistance loci, which are controlled by a dominant/recessive relationship. The  Yr1 ,  Yr6 ,  Yr9 ,  Yr17 ,  Yr27 ,  Yr25 ,  Yr28 ,  Yr29 ,  Yr32 ,  YrTr1  and  YrSP resistance loci are governed by two complementary dominant/recessive genes. Avirulence against heterozygous  Yr4 ,  Yr43Yr44 ,  Yr76  and  YrExp2  resistance loci is regulated by a dominant and recessive, or a dominant and suppressor gene pairs. Totally, 117 multi-locus genotypes (MLGs) were detected at 24 KASP-SNP marker loci among the 120 progeny. Using these marker loci, we constructed a linkage map with a genetic distance interval spanning 624.5 cM. Quantitative trait loci (QTL) corresponding to phenotypic segregation for virulence at 20  Yr  resistance loci in addition to the  Yr1  resistance locus were identified. These results facilitate our understanding of Pst virulence evolution, and simplify breeding of wheat cultivars with effective resistance to wheat stripe rust.

原文链接:https://doi.org/10.1094/PDIS-07-22-1637-RE

Baidu
sogou