论文信息：Xueting Chen , Yuanyuan Wang , Wenjun Wu , Ke Dong , Zhaonong Hu. DSC1 channel-dependent developmental regulation of pyrethroid susceptibility in Drosophila melanogaster.Pesticide Biochemistry and Physiology 148 (2018) 190–198
JCR分区Q1，中科院大类三区，IF=3.44
论文摘要：Pyrethroid insecticides modify the gating of voltage-gated sodium channels, thus disrupting the function of thenervous system. In Drosophila melanogaster, para encodes a functional sodium channel. Drosophila Sodium Channel 1 (DSC1), although considered as a putative sodium channel gene for decades due to its high sequence similarity with sodium channels, encodes a voltage-gated cation channel with high permeability to Ca 2+ . Previous study showed that knockout of the DSC1 gene (DSC1 −/− ) caused Drosophila adults to be more susceptible to pyre-throids and the adult giant fiber (GF) neural circuit were more susceptible to pyrethroids. Considering distinct expression of DSC1 transcripts in adults and larvae, we examined the role of DSC1 channels in regulating pyrethroid susceptibility in Drosophila larvae. We conducted insecticide bioassays and examined the suscept- ibility of the larval neuromuscular junction (NMJ) to pyrethroids using w 1118 , an insecticide-susceptible line,DSC1 −/− , para ts1 (a pyrethroid-resistant line carrying a mutation in para) and a double mutation line para ts1 ;DSC1 −/− . We found that, like the adult GF system, the NMJ of DSC1 −/− flies is more susceptible to pyrethroids than that of w 1118 with the pyrethroid susceptibility ranked as DSC1 −/− > w 1118 > para ts1 ; DSC1 −/−> para ts1 . However, DSC1 −/− larvae were about two-fold more resistant to pyrethroids than w 1118 larvae, and the pyrethroid susceptibility of larvae ranked as w 1118 > DSC1 −/− > para ts1 ; DSC1 −/− > para ts1 . These results reveal common and distinct roles of DSC1 channels in regulating the action of pyrethroids in adults and larvae of D. melanogaster.
